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KMID : 0603519970020020155
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Journal of Korean Association of Cancer Prevention
1997 Volume.2 No. 2 p.155 ~ p.161
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Comparison of the Inhibitory Effects of Retinoic Acid Derivatives on Development of Squamous Metaplasia from Rat Mammary Epithelial Organoids Cultured in Matrigel
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Paik Kee-Joo
Jeon Seong-Sill
Yoon Jeong-Hyun
Kim Nam-Deuk
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Abstract
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To compare the inhibitory effects of retinoic acids derivatives among all-tracts, 13-cis and 9-cis retinoic acids on the development of squamous metaplasia (SQM) from rat mammary epithelial cells (RMEC), female F344 rat mammary organoids were cultured in reconstituted basement membrane, Matrigel, under either a complete hormone medium (CHM) or serum-free mammary epithelium growth medium (MEGM). From the culture, five different types of multicellular colonies were developed: stellate, ductal, webbed, squamous, and lobulo-ductal colonies. Organoids cultured in CHM without retinoids gave rise to fewer such SQM (∼5%) than those in MEGM without retinoids (∼16%). Formation of SQM was completely suppressed when three different retinoic acids derivatives [10-9 M], all-trans, 13-cis and 9-cis RAs, were added to CHM. However, a few SQM were still observed in cultures in MEGM with the added retinoids [10-6 M]. In the presence of retinoids in culture medium, lobulo-ductal colonies, one of well-differentiated structures, were increased with the augmentation of retinoids concentrations. However, there were no difference in the differentiation inducing activities among three different retinoic acid derivatives.
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KEYWORD
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Squamous metaplasia, all-trans retinoic acid, 13-cis retinoic acid, 9-cis retinoic acid
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